Biochem/physiol Actions

Primary TargetA highly sensitive, photostable caspase-3 fluorogenic substrate

Cell permeable: no

Reversible: no

Product does not compete with ATP.

General description

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Useful for high throughput screening applications.

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hug, H., et al. 1999. Biochemistry38, 13906.Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

Warning

Toxicity: Standard Handling (A)